Transposon mutagenesis in Acinetobacter calcoaceticus RAG-1
نویسندگان
چکیده
منابع مشابه
Self-Cycling Fermentation Applied to Acinetobacter calcoaceticus RAG-1.
The self-cycling fermentation (SCF) technique was applied to a culture of Acinetobacter calcoaceticus RAG-1. This method was shown to result in synchronization of the cells, achieving a 77% improvement in cell synchrony over that of the batch case. Cellular occurrences, averaged out by asynchronous batch cultures, were magnified by the temporal alignment of metabolic events brought about by the...
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When Acinetobacter calcoaceticus RAG-1 was grown together with an emulsan-deficient mutant on crude oil, only the emulsan-producing RAG-1 was found to grow, regardless of whether the medium was supplemented with emulsan. The results suggested that the cell-associated form of the bioemulsifier is the biologically active species required for growth on crude oil. A revertant of an emulsan-deficien...
متن کاملThe isolation of cell surface mutants of Acinetobacter calcoaceticus RAG-1.
The isolation of cell surface mutants of Acinetobacter calcoaceticus RAG-1 was demonstrated by a method based on the differential immunoprecipitating properties of wild-type cells and mutants. In the presence of antiserum, about 99% of wild-type cells in suspension sedimented following overnight agglutination. The kinetics of antiserum-induced agglutination was quantified by measuring the time-...
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Serotyping of Acinetobacter calcoaceticus by direct immunofluorescence and a capsule swelling reaction is described. One hundred isolates, including 12 from an outbreak in a neonatal department, were studied. Ninety five per cent of the isolates were typable by immunofluorescence and could be divided into 30 separate types, but 42.1% of typable strains, including 11 from the outbreak, were of o...
متن کاملChromosome mapping in Acinetobacter calcoaceticus.
RP4-mediated conjugation was used to map the loci of 23 different mutations on a circular linkage group in Acinetobacter calcoaceticus EBF65/65. The resulting genetic map indicated that the chromosomal organization of A. calcoaceticus differed from that of members of the enteric group of bacteria and was similar to Pseudomonas in showing an absence of clustering of functionally related genes.
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1993
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.175.6.1838-1840.1993